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منتج #:
145284746
NP40 Cell Lysis Buffer (2X), 7.4 pH, 250mL
منتج #:
145284746
KWD 21
تفاصيل السعر
باستثناء رسوم الشحن والجمارك ( سيتم احتساب رسوم الشحن والجمارك عند إتمام الشراء )
*سيتم استيراد جميع العناصر من أمريكا
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اكتب رأيك
متوفر فى المخزون
مستورد من متجر USA
كمية:
اطلب الآن واحصل عليه حول الخميس, سبتمبر 10
معاملة آمنة
تعمل يوباي جاهدة لحماية أمنك وخصوصيتك. يضمن نظام أمان الدفع المتقدم لدينا السرية من خلال تشفير معلوماتك أثناء النقل باستخدام بروتوكولات AES (معايير التشفير المتقدمة) وSSL (طبقة المنافذ الآمنة). تفاصيل الدفع الخاصة بك آمنة بنسبة %100 لأننا لا نشارك تفاصيل الدفع الخاصة بك مع بائعين تابعين لجهات خارجية
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Manufactured in an ISO 9001 and 13485 certified facility in the USA, ensuring the highest quality for your research needs.
شحن
سريع
استرجاع
مجاني*
تغليف آمن
منتجات أصلية %100
الامتثال لمعيار PCI DSS
حاصل على شهادة ISO 27001
Note: Step Down Voltage Transformer required for using electronics products of أمريكا store (110-120). Recommended power converters اشتري الآن.
مايفيد
Dual Concentration
The NP40 Cell Lysis Buffer is formulated at a 2X concentration, ensuring efficient and effective cell lysis in fewer steps, saving time for researchers while maintaining optimal results.
pH Stability
Maintaining a pH of 7.4, this buffer provides a controlled environment, crucial for preserving protein integrity during lysis, making it ideal for sensitive experiments and applications.
Generous Volume
With 250mL of solution, there is ample buffer for numerous applications, reducing the frequency of repurchasing and ensuring that labs can perform multiple experiments seamlessly.
تفاصيل المنتج
| Container Type | Bottle |
| Item Form | Liquid |
| Item Weight | 4 ounces (113.4 grams) |
| Volume | 250 ml |
| Brand Name | eCon Lab Supply |
| Manufacturer | Ciro Mfg |
| Included Components | Bottle |
| Part Number | 23.105.112 |
| Brand | eCon Lab Supply |
| Item Volume | 250 ml |
| Package Information | Bottle |
ما بداخل الصندوق
Bottle
من يجب أن يشتري؟
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Molecular Biologists
Ideal for extracting proteins from cells to facilitate downstream molecular biology applications.
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Biochemistry Laboratories
Useful in enzymatic studies where cell lysis is needed to release cellular components.
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Research Institutions
Beneficial for researchers conducting studies on cellular processes and protein interactions.
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General Consumers
Not suitable for non-laboratory individuals due to specialized usage and safety considerations.
وصف المنتج
هل لديك أي استفسار؟ تحدث معنا
أسئلة العملاء & الإجابات
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سؤال:
What is NP40 Cell Lysis Buffer and how is it used?
إجابه: NP40 Cell Lysis Buffer is a specialized solution designed for the efficient lysis of cells to extract cellular components such as proteins and nucleic acids. It operates at a neutral pH of 7.4 and is recommended for use in cell biology experiments where high yield and purity are critical. This buffer can disrupt both cell membranes and nuclear membranes, effectively releasing contents for further analysis. It is widely used in research laboratories for applications like Western blotting, immunoprecipitation, and enzyme assays. -
سؤال:
What are the key components of NP40 Cell Lysis Buffer?
إجابه: The NP40 Cell Lysis Buffer contains Nonidet P-40 (NP-40), a non-ionic detergent, along with salts and buffering agents that maintain the pH at 7.4. These components work synergistically to solubilize membrane proteins without denaturing them, which is crucial for downstream applications like SDS-PAGE or mass spectrometry. The balance of these ingredients is tailored to provide optimal lysis conditions while preserving protein functionality, making it a popular choice for life science research. -
سؤال:
Can NP40 Cell Lysis Buffer be used for different cell types?
إجابه: Yes, NP40 Cell Lysis Buffer is versatile and can be applied to various cell types, including mammalian, yeast, and bacterial cells. Its formulation allows for efficient lysis regardless of the cellular composition. This makes it an excellent tool in diverse experimental setups, such as studying protein interactions in mammalian cells or extracting plasmids from bacterial cells. However, it's recommended to optimize conditions based on specific cell types and experimental goals. -
سؤال:
How should NP40 Cell Lysis Buffer be stored?
إجابه: NP40 Cell Lysis Buffer should be stored at 4°C to maintain its stability and effectiveness. For longer storage, aliquoting the buffer into smaller tubes can help minimize repeated freeze-thaw cycles, which may compromise its integrity. Always check for any signs of precipitation or discoloration before use. Proper storage extends its viability, ensuring consistent performance in experiments over time, especially in high-throughput applications. -
سؤال:
What precautions should be taken while using NP40 Cell Lysis Buffer?
إجابه: When using NP40 Cell Lysis Buffer, it's important to wear appropriate personal protective equipment, such as gloves and goggles, to prevent skin and eye contact with the buffer. Additionally, work in a well-ventilated area to avoid inhaling vapors. Ensure that all reagents are at the correct temperature prior to use and that your lab solutions are prepared under sterile conditions to prevent contamination. These precautions ensure a safe and controlled experimental environment. -
سؤال:
How does NP40 Cell Lysis Buffer affect protein assays?
إجابه: NP40 Cell Lysis Buffer is designed to maintain protein stability while extracting them from cells. It minimizes protein denaturation, which is crucial for assays such as Western blotting or ELISA. This buffer allows for the recovery of functional proteins, making it highly suitable for assays where protein activity is determined. Users often find that using this buffer leads to more consistent and reproducible results in their analyses. -
سؤال:
Is NP40 Cell Lysis Buffer suitable for DNA extraction?
إجابه: While NP40 Cell Lysis Buffer is primarily developed for protein extraction, it can also aid in DNA extraction by breaking down cell membranes that house both proteins and nucleic acids. However, to ensure purity, it's advisable to perform a phenol-chloroform extraction following lysis. This approach allows for the recovery of high-quality DNA suitable for applications such as PCR. Researchers often use this buffer to facilitate simultaneous protein and DNA study within the same sample. -
سؤال:
Are there any alternatives to NP40 Cell Lysis Buffer?
إجابه: Yes, there are several alternatives to NP40 Cell Lysis Buffer, including Triton X-100 and SDS-based lysis buffers. Each of these alternatives may have specific advantages depending on the application. For instance, Triton X-100 is milder and less likely to disrupt protein structure, making it suitable for applications requiring intact proteins. In contrast, SDS is more effective for complete denaturation of proteins. Researchers often choose a buffer based on their specific experimental needs and the type of analysis they plan to perform. -
سؤال:
How much NP40 Cell Lysis Buffer should I use per sample?
إجابه: The optimal volume of NP40 Cell Lysis Buffer typically depends on the type and number of cells being lysed. A general recommendation is to use 100-300 µL of the buffer per 1-10 million cells. Adjusting the volume may be necessary based on cell density and the specific requirements of your assay. Researchers find that using the right amount ensures efficient lysis while preventing the dilution of key cellular components, leading to more reliable experimental outcomes. -
سؤال:
Where can I buy NP40 Cell Lysis Buffer (2X), 7.4 pH, 250mL in Kuwait?
إجابه: You can purchase NP40 Cell Lysis Buffer (2X), 7.4 pH, 250mL from Ubuy in Kuwait. Ubuy offers a reliable platform for sourcing laboratory reagents and supplies, making it a convenient choice for researchers and scientists needing high-quality buffers and reagents. Ensure you check their website for availability and details on your specific needs.
eCon Lab Supply Buffers Editorial Review
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منصة موثوقة وثقة كاملة للمشتري
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Ahmad
مشتري موثّق
“Fast delivery, great experience overall.”
03-Jul-26 · عبر Trustpilot
AM
abbas
مشتري موثّق
“Excellent item received as expected and before the estimated time. Thanks for the great service.”
19-Jul-26 · عبر Trustpilot
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Waleed
مشتري موثّق
“Thank you for your message. I received the product and it is very good ? I?m really impressed with how great it works! I will be happy to share my feedback on the website.”
11 February 2026 · عبر Trustpilot
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Ahmed
مشتري موثّق
“Very good the same exact product that i wanted good shipping and delivery”
10 June 2026 · عبر Trustpilot
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Ambhita
مشتري موثّق
“Package delivered on time”
10 July 2026 · عبر Trustpilot
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منتجات أصلية
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معلومات مهمة
- القيود: بالنسبة للمنتجات التي يتم شحنها دولياً، يُرجى ملاحظة أن أي ضمان من الشركة المصنعة قد لا يكون صالحاً؛ قد لا تتوفر خيارات خدمة الشركة المصنعة؛ قد لا تكون أدلة المنتج والتعليمات وتحذيرات السلامة مكتوبة بلغة بلد المقصد؛ قد لا يتم تصميم المنتجات (والمواد المصاحبة لها) وفقاً لمعايير بلد الوجهة والمواصفات ومتطلبات الملصقات؛ وقد لا تتوافق المنتجات مع الجهد الكهربي المستخدم في بلد الوجهة والمعايير الكهربائية الأخرى (تتطلب استخدام محوّل كهربي أو جهاز تحويل إذا كان ذلك مناسباً). المستلم مسؤول عن ضمان إمكانية استيراد المنتج بشكل قانوني إلى بلد الوجهة. عند الطلب من يوباي أو الشركات التابعة لها، يكون المستلم هو المستورد المسجل ويجب أن يلتزم بجميع القوانين واللوائح الخاصة ببلد الوجهة.
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KWD 21
اطلب الآن واحصل عليه حول الخميس, سبتمبر 10
هذا المنتج غير ممنوع في بلدي. (الرجاء الضغط على الرابط أعلاه إذا لم يكن هذا المنتج ممنوعاً في بلدك ، لذلك سيقوم فريقنا بمراجعته والسماح به.)
كمية:
معاملة آمنة
نوفر لك مدفوعات مشفّرة، وحماية متكاملة للمشتري، مع الالتزام بمعايير PCI DSS وشهادة ISO 27001:2022 لضمان أعلى مستويات الأمان في كل عملية شراء.
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المميزات والفوائد
- Comprehensive range of biological buffers.
- Ideal for research, diagnostics, drug development, and manufacturing.
- Certified facility guarantees top-tier quality.
- Designed for protein research and molecular biology.
- NP40 Cell Lysis Buffer is suitable for key assays like ELISA and Western blotting.
- Strict adherence to Good Manufacturing Practices ensures reliability.
ضمان Ubuy
تسوّق بثقة مع منتجات أصلية %100، ومدفوعات آمنة متوافقة مع معيار PCI DSS، وحماية بيانات معتمدة وفق ISO 27001، وشحن دولي سريع، وإرجاع مجاني*، وتغليف آمن لكل طلب.